The endophytic potential, Bacillus spp. for controlling Meloidogyne sp. and increasing tomato growth and production

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Winarto
Yulmira Yanti
Hasmiandy Hamid
Yaherwandi

Abstract

Meloidogyne spp. is responsible of root swelling, one of the primary disease in tomato plants. Controlling this nematode is challenging due to its wide host range. The use of synthetic nematicides harms the environment; therefore, alternative controls, such as biological methods, are necessary. Among the biological agents, one group includes endophytic bacteria that reside in plant tissues and do not cause harm to plants. These bacteria enhance plant resistance to pests and pathogens while promoting plant growth. The study aimed to acquire endophytes, Bacillus spp. strains capable of controlling Meloidogyne sp. while stimulating the growth of tomato plants. The research employed a completely randomized design (CRD) comprising seven treatments and five replications. The treatments consisted of B. cereus strain SNE 2.2, TLE 2.3 and TLE 1.1, B. pseudomycoides strain EPL 1.1.3, B. toyonensis strain EPL 1.1.4, positive control (without the introduction of Bacillus spp. and inoculation with Meloidogyne sp.) and negative control (without Bacillus spp. and without Meloidogyne sp.). Bacillus spp. endophyites were introduced in two stages: into the seeds and into the roots of tomato seedlings for 15 min. The observed variables were the development of Meloidogyne sp., endophytic colonization of Bacillus spp., and plant growth. The results demonstrated that all Bacillus spp. were effective in controlling Meloidogyne sp. and enhancing the growth of tomato plants. The best isolate in controlling Meloidogyne sp. and increasing the growth of tomato plants was B. cereus strain SNE2.2.

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Winarto; Yanti, Y.; Hamid, H. .; Yaherwandi. The Endophytic Potential, Bacillus Spp. for Controlling Meloidogyne Sp. And Increasing Tomato Growth and Production. J Trop Plant Pests Dis 2024, 24, 66-74.


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References

Ashoub AH & Amara MT. 2010. Biocontrol activity of some bacterial genera against root-knot nematode, Meloidogyne incognita. J. Am. Sci. 6(10): 321–328.

Chawla G, Singh KP, & Srivastava AN. 2006. Study on propagation of root knot nematode, Meloidogyne incognita (Kofoid & White) Chitwood, through tuberose (Polianthes tuberosa Linn.) bulbs. Journal of Ornamental Horticulture. 9(1): 73–74.

Fang Y & Ramasamy RP. 2015. Current and prospective methods for plant disease detection. Biosensors (Basel). 5(3): 537-561. https://doi.org/10.3390/bios5030537

Fitriani HP & Haryanti S. 2016. Pengaruh penggunaan pupuk nanosilika terhadap pertumbuhan tanaman tomat (Solanum lycopersicum) var. bulat [Effect of nanosilica fertilizer on plant growth of tomato (Solanum lycopersicum) var. bulat]. Buletin Anatomi dan Fisiologi. 24(1): 34–41.

Habazar T, Winarto, Obel, Yanti Y, Dani MR, & Monica D. 2021. Biocontrol of Meloidogyne sp. on tomato plants by selected Bacillus spp. IOP Conf. Ser.: Earth Environ. Sci. 757: 012019. https://doi.org/10.1088/1755-1315/757/1/012019

Hallmann J, Quadt-Hallmann A, Mahaffee WF, & Kloepper JW. 1997. Bacterial endophytes in agricultural crops. Can. J. Microbiol. 43(10): 895–914. https://doi.org/10.1139/m97-131

Hallmann J. 2001. Plant interactions with endophytic bacteria. In: Jeger MJ & Spence NJ (Eds.). Biotic Interactions in Plant Pathogen Associations. pp. 87–119. CABI Publishing, Wallingford. https://doi.org/10.1079/9780851995120.0087

Harni R & Samsudin. 2015. Pengaruh formula bionematisida bakteri endofit Bacillus sp. terhadap infeksi nematoda Meloidogyne sp. pada tanaman kopi [Effect of endophytic bionematicide Bacillus sp. on the infection of Meloidogyne sp. of coffee plants]. J. TIDP. 2(3): 143–150.

Harni R. 2014. Prospek penggunaan bakteri endofit untuk pengendalian nematoda Pratylenchus brachyurus pada tanaman nilam [Prospects for using endophytic bacteria to control Pratylenchus brachyurus nematode in patchouli plants]. Perspektif: Review Penelitian Tanaman Industri. 13(1): 1–12.

Hrynkiewiezc K, & Baum C. 2012. The potential of rhizosphere microorgamisms to promote the plant growth in disturbed soils. In: Malik A., & Grohmann (Eds). Environmental protection strategies for sustainable development. USA

Hussey RS & Barker KR. 1973. A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Disease Reporter. 57(12): 1025–1028.

Khan AR, Mustafa A, Hyder S, Valipour M, Rizvi ZF, Gondal AS, Yousuf Z, Iqbal R, & Daraz U. 2022. Bacillus spp. as bioagents: uses and application for sustainable agriculture. Biology. 11(12): 1763. https://doi.org/10.3390/biology11121763

Kuklinsky-Sobral J, Araújo WL, Mendes R, Geraldi IO, Pizzirani-Kleiner AA, & Azevedo JL. 2004. Isolation and characterization of soybean associated bacteria and their potential for plant growth promotion. Environ. Microbiol. 6(12): 1244–1251. https://doi.org/10.1111/j.1462-2920.2004.00658.x

Mekete T, Hallmann J, Kiewnick S, & Sikora R. 2009. Endophytic bacteria from Ethiopian coffee plants and their potential to antagonise Meloidogyne incognita. Nematology. 11(1): 117-127. https://doi.org/10.1163/156854108X398462

Miljakovi? D, Marinkovi? J, & Baleševi?-Tubi? S. 2020. The significance of Bacillus spp. in disease suppression and growth promotion of field and vegetable crops. Microorganisms. 8(7): 1037. https://doi.org/10.3390/microorganisms8071037

Munif A, Hallmann J, & Sikora RA. 2000. Evaluation of the biocontrol activity of endophytic bacteria from tomato against Meloidogyne incognita. Proceedings of the Mededelingen Faculteit Landbouwkundige En Toegepaste Biologische Wetenschappen, Vol. 65. pp. 471–480. Universiteit Gent. Belgium.

Pratiwi NWK, Amrulloh R, Auly FE, & Kurniawati F. 2020. Deteksi dan identifikasi nematoda puru akar (Meloidogyne spp.) pada tanaman bit menggunakan metode DNA barcoding [Detection and identification root-knot nematode (Meloidogyne spp.) in sugar beet using DNA barcoding method]. Jurnal Fitopatologi Indonesia. 16(1): 1–8. https://doi.org/10.14692/jfi.16.1.1-8

Rao MS, Dwivedi MK, Kumar RM, Chaya MK, Rathnamma K, Rajinikanth R, Grace GN, Priti K, Shree NV, Kamalnath M, Prabu P, Krishna CG, Rini P, & Shivananda TN. 2014. Evaluation of bio-efficacy of Bacillus subtilis (NBAIMCC-B- 01211) against disease complex caused by Meloidogyne incognita and Fusarium oxysporum f.sp. vasinfectum in okra. Pest Manage. Hortic.Ecsyst. 20(2): 217–221.

Siddiqui ZA, Shehzad M, & Alam S. 2014. Interactions of Ralstonia solanacearum and Pectobacterium carotovorum with Meloidogyne incognita on potato. Arch. Phytopathol. Plant Prot. 47(4): 449–455. https://doi.org/10.1080/03235408.2013.811810

Sikora RA & Fernández E. 2005. Nematode parasites of vegetables. In: Luc M, Sikora RA, & Bridge J (eds). Plant Parasitic Nematodes in Subtropical and Tropical Agriculture. 2nd Edition. pp 319-392. CABI Publishing, Wallingford. https://doi.org/10.1079/9780851997278.0319

Sikora RA, Schäfer K, & Dababat AA. 2007. Modes of action associated with microbially induced in planta suppression of plant-parasitic nematodes. Australas. Plant Pathol. 36(2): 124–134. https://doi.org/10.1071/AP07008

Sturz AV, Christie BR, & Nowak J. 2000. Bacterial endophytes: Potential role in developing sustainable systems of crop production. Crit. Rev. Plant Sci. 19(1): 1–30. https://doi.org/10.1016/S0735-2689(01)80001-0

Suryaningsih E. 2008. Pengendalian penyakit sayuran yang ditanam dengan sistem budidaya pada pertanian periurban [Disease control method for several vegetables planted in mosaic farming system in periurban agriculture]. J. Hort. 18(2): 200–211.

Tian XL, Zhao XM, Zhao SY, Zhao JL, & Mao ZC. 2022. The biocontrol functions of Bacillus velezensis strain Bv-25 against Meloidogyne incognita. Front. Microbiol. 13: 843041. https://doi.org/10.3389/fmicb.2022.843041

Thokchom E, Thakuria D, Kalita MC, Sharma CK, & Talukdar NC. 2017. Root colonization by host-specific rhizobacteria alters indigenous root endophyte and rhizosphere soil bacterial communities and promotes the growth of mandarin orange. Eur. J. Soil Biol. 79: 48–56. https://doi.org/10.1016/j.ejsobi.2017.02.003

Wulandari D, Sulistyowati L, & Muhibuddin A. 2014. Keaneakaragaman jamur endofit pada tanaman tomat (Lycopersicum esculentum Mill.) dan kemampuan antagonisnya terhadap Phytophthora infestans [Diversity of endophytic fungi in tomato (Lycopersicum esculentum Mill.) plants and their antagonistic ability against Phytophthora infestans]. Jurnal HPT (Hama Penyakit Tumbuhan). 2(1): 110–118.

Yanti Y, Habazar T, & Resti Z. 2017. Formulasi padat rhizobakteria indigenus Bacillus thuringiensis TS2 dan waktu penyimpanan untuk mengendalikan penyakit pustul bakteri Xanthomonas axonopodis pv. glycines [Solid formulation of indigenous rhizobacteria Bacillus thuringiensis TS2 and storage time to control bacterial pustule disease Xanthomonas axonopodis pv. glycines]. J. Trop. Plant Pests Dis. 17(1): 9–18. https://doi.org/10.23960/j.hptt.1179-18

Yanti Y, Hamid H, & Reflin R. 2019. Indigenous rhizobacteria screening from tomato to control Ralstonia syzigii subsp. indonesiensis and promote plant growth rate and yield. J. Trop. Plant Pests Dis. 18(2): 177–185. https://doi.org/10.23960/j.hptt.218177-185